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superscript® iii reverse transcriptase synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscript® iii reverse transcriptase synthesis kit
    Superscript® Iii Reverse Transcriptase Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+-mmlv+reverse+transcriptase/superscript++iii+reverse+transcriptase+synthesis+kit/pmc12221670-72-18-24
    Average 90 stars, based on 1 article reviews
    superscript® iii reverse transcriptase synthesis kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: NKG2D receptor ligands are cell surface biomarkers for injured murine and human nociceptive sensory neurons
    Article Snippet: Samples were heated to 65°C for 5 min and cooled on ice before the addition of reaction buffer containing reverse-transcriptase (Superscript III, Invitrogen, Life Technologies). cDNA was synthesised on a thermo-cycler (Biorad T100) (25°C, 5 min; 50°C, 100 min; 85°C, 5 min) and finally 37°C for 20 min in the presence of RNase H. cDNA was stored at −20°C.

    Article Title: p75 NTR regulates postnatal skeletal development via NGF-responsive JNK signaling
    Article Snippet: Total RNAs of tissues and cells were harvested using RNeasy mini kit (Qiagen, Hilden, Germany) and reverse-transcribed using SuperScript® III Reverse Transcriptase Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA).

    Reverse Transcription:

    Article Title: <i>In vivo</i> and <i>ex vivo</i> cardiovascular haemodynamic responses of preterm growth-restricted lambs to perinatal asphyxia
    Article Snippet: .. Total RNA was extracted from 20 to 30 mg of frozen left ventricular myocardium (RNeasy Mini Kit, Qiagen) before 50 ng/μl of RNA was reverse-transcribed into complementary DNA (cDNA, SuperScript III Reverse Transcriptase, Thermo Fisher). .. Relative mRNA levels of α1-AR, β1-AR, atrial natriuretic peptide (NPPA), B-type natriuretic peptide (NPPB) and sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA/ATP2A2) (Table 1) were measured by RT-qPCR (QuantStudio 6 Real-Time PCR system, ThermoFisher, U.S.A.).

    Article Title: B lymphocytes that enter the germinal center late preferentially differentiate into memory cells that recognize subdominant epitopes
    Article Snippet: The RNA was eluted from the beads with 11 uL of a solution containing random primers (14.5 ng/uL, Invitrogen, 48190-011), tergitol (0.5% NP-40 70% in H2O, Sigma-Aldrich, NP40S), and RNase inhibitor (0.6 U/uL, Promega, N2615) in nuclease-free water (Qiagen), and then incubated at 65 °C for 3 minutes. .. Subsequently, cDNA was synthesized by reverse transcription using 7 uL of a solution containing SuperScript III Reverse Transcriptase, 5× buffer, dNTPs (25 mM), DTT (SuperScript III Reverse Transcriptase, Invitrogen, 18080-044, 10,000 U), and RNase inhibitor (0.6 U/uL, Promega, N2615) in nuclease-free water (Qiagen). ..

    Article Title: Epitranscriptomic Modulation of TET2 Inhibition Suppressed SARS-CoV-2 Infection and Blocked Viral Nucleocapsid Protein in Induced-Pluripotent-Stem-Cell-Derived Cardiomyocyte Screening Models
    Article Snippet: .. Following previous protocols [ ], complementary DNA synthesis was carried out using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) with 500 to 1,000 ng of total RNA as input. ..

    Article Title: Single-cell RNA sequencing clarifies dermal fibroblast subset representation in vitro and reveals variable persistence of keloid disease-associated features
    Article Snippet: .. RNA was reverse transcribed using SuperScript III reverse transcriptase (200U/μL) with dithiothreitol (0.1M) and first-strand buffer (5X) (Invitrogen, #18080093) and random primers (Invitrogen, #48190011) plus dNTPs (Invitrogen, #18427013) according to manufacturer’s instructions. .. Due to low RNA yield (<4ng/μL) from some samples, RNA from all samples for the P0-P4 sequencing validation experiment were amplified and converted to cDNA using the Smart-seq2 protocol (steps 1 to 27) ( ). cDNA was diluted to 2ng/μL, and qPCR was performed using a Roche LightCycler 480II with SYBR Green I (2x; Roche, #04887352001) according to manufacturer’s instructions.

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells
    Article Snippet: .. 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044. .. SuperScript III Reverse Transcriptase , Thermo Fisher Scientific , Cat#18080044.

    Synthesized:

    Article Title: B lymphocytes that enter the germinal center late preferentially differentiate into memory cells that recognize subdominant epitopes
    Article Snippet: The RNA was eluted from the beads with 11 uL of a solution containing random primers (14.5 ng/uL, Invitrogen, 48190-011), tergitol (0.5% NP-40 70% in H2O, Sigma-Aldrich, NP40S), and RNase inhibitor (0.6 U/uL, Promega, N2615) in nuclease-free water (Qiagen), and then incubated at 65 °C for 3 minutes. .. Subsequently, cDNA was synthesized by reverse transcription using 7 uL of a solution containing SuperScript III Reverse Transcriptase, 5× buffer, dNTPs (25 mM), DTT (SuperScript III Reverse Transcriptase, Invitrogen, 18080-044, 10,000 U), and RNase inhibitor (0.6 U/uL, Promega, N2615) in nuclease-free water (Qiagen). ..

    DNA Synthesis:

    Article Title: Epitranscriptomic Modulation of TET2 Inhibition Suppressed SARS-CoV-2 Infection and Blocked Viral Nucleocapsid Protein in Induced-Pluripotent-Stem-Cell-Derived Cardiomyocyte Screening Models
    Article Snippet: .. Following previous protocols [ ], complementary DNA synthesis was carried out using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) with 500 to 1,000 ng of total RNA as input. ..

    Generated:

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [ , ]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Sequencing:

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [ , ]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).



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